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epifluorescence inverted microscope  (Nikon)


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    Structured Review

    Nikon epifluorescence inverted microscope
    Epifluorescence Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+epifluorescence+microscope+eclipse+ti2/ECLIPSE+Ti2/pm41894427-71-8-11
    Average 99 stars, based on 11764 article reviews
    epifluorescence inverted microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Inverted Epifluorescence:

    Article Title: Event-based optical imaging and reconstruction of in vivo neuronal and vascular dynamics
    Article Snippet: .. Event camera optical setup The event camera (DVXplorer, iniVation) was mounted on the left port of an inverted epifluorescence microscope equipped with a motorized filter wheel (Eclipse Ti2, Nikon; Fig. 1). .. An sCMOS camera (Kinetix22, Teledyne Photometrics) was attached to the eyepiece port to enable simultaneous fluorescence imaging.

    Article Title: A tumor-resident bacterium promotes cervical cancer progression through lysoPC-mediated c-Jun/c-Fos activation.
    Article Snippet: Two additional 5-min washes with 2× SSC buffer were performed, followed by rinsing with a wash buffer containing (25% formamide (Ambion, AM9342), 2× SSC, and nuclease-free water) and a final 5-min incubation in fresh wash buffer at RT. .. Probes were then diluted to 2 ng/mL in hybridization buffer (25% formamide, 10% dextran sulfate (Sigma-Aldrich, D8906), 1 mg/mL E. coli tRNA (Sigma-Aldrich, R4251), 2× SSC, and 0.02% BSA (Ambio, AM2616)) and hybridized to tissues overnight at 37 ◦ C. Unbound probes were removed by washing with the same buffer for 30 min at 37 ◦ C. Nuclei were counterstained with 2 ng/mL DAPI (Sigma-Aldrich, D9564) for 5 min at 30 ◦ C. Finally, samples were mounted with Antifade Mounting Medium (Beyotime, P0126) and imaged using an inverted epifluorescence microscope (Nikon Eclipse Ti2). ..

    Article Title: mnDINO: Accurate and robust segmentation of micronuclei with vision transformer networks
    Article Snippet: .. MNFinder images are taken from both Leica DMi8 laser scanning confocal microscope and Leica DMi8 with Adaptive Focus widefield microscope at 20x and 40x resolutions. mnDINO data subset 01 is generated from a Nikon Ti2 Eclipse inverted epifluorescence microscope, equipped with a IRIS9 camera, a 20X objective (Plan Apochromat, NA 0.8) and a 0.7X demagnifying C-Mount adaptor using HeLa cells, resulting in 18 high-resolution images of 2960x2960 pixels. .. 1,688 ground truth objects were manually annotated. mnDINO data subset 02 comes from a Nikon Ti2 Eclipse epifluorescence microscope, equipped with a Kinetix camera and a 20x objective (Plan Apochromat, NA 0.8) on HeLa and RPE1 p53-/- cells, where 6 high-resolution images at 2720x2720 pixels were annotated with 582 objects.

    Hybridization:

    Article Title: A tumor-resident bacterium promotes cervical cancer progression through lysoPC-mediated c-Jun/c-Fos activation.
    Article Snippet: Two additional 5-min washes with 2× SSC buffer were performed, followed by rinsing with a wash buffer containing (25% formamide (Ambion, AM9342), 2× SSC, and nuclease-free water) and a final 5-min incubation in fresh wash buffer at RT. .. Probes were then diluted to 2 ng/mL in hybridization buffer (25% formamide, 10% dextran sulfate (Sigma-Aldrich, D8906), 1 mg/mL E. coli tRNA (Sigma-Aldrich, R4251), 2× SSC, and 0.02% BSA (Ambio, AM2616)) and hybridized to tissues overnight at 37 ◦ C. Unbound probes were removed by washing with the same buffer for 30 min at 37 ◦ C. Nuclei were counterstained with 2 ng/mL DAPI (Sigma-Aldrich, D9564) for 5 min at 30 ◦ C. Finally, samples were mounted with Antifade Mounting Medium (Beyotime, P0126) and imaged using an inverted epifluorescence microscope (Nikon Eclipse Ti2). ..

    Microscopy:

    Article Title: Listeria-infected macrophages promote biomechanical alterations in endothelial cell monolayers for transmigration.
    Article Snippet: Thirty minutes before the TFM recording, cell culture media was changed to L-15 CO 2 -independent medium supplemented with EGMTM-2 BulletKit Medium (MEDIUM CC-3156 and Kit CC-4176, Lonza). .. Samples in the 24-well plate were then transferred to the insert stage of an inverted Nikon Eclipse Ti2 epifluorescence microscope equipped with a Prime 95B camera for imaging. .. Note that for experiments involving addition of recombinant TNF-α (10 ng/mL, DL20083053, Invivogen) or anti-TNF-α antibody (1 μg/mL, MAB9677, Bio-techne) or isotype control (1 μg/mL, DNP-M2, ACROBiosytems), treatments were applied at the same time as MΦ.

    Article Title: mnDINO: Accurate and robust segmentation of micronuclei with vision transformer networks
    Article Snippet: .. MNFinder images are taken from both Leica DMi8 laser scanning confocal microscope and Leica DMi8 with Adaptive Focus widefield microscope at 20x and 40x resolutions. mnDINO data subset 01 is generated from a Nikon Ti2 Eclipse inverted epifluorescence microscope, equipped with a IRIS9 camera, a 20X objective (Plan Apochromat, NA 0.8) and a 0.7X demagnifying C-Mount adaptor using HeLa cells, resulting in 18 high-resolution images of 2960x2960 pixels. .. 1,688 ground truth objects were manually annotated. mnDINO data subset 02 comes from a Nikon Ti2 Eclipse epifluorescence microscope, equipped with a Kinetix camera and a 20x objective (Plan Apochromat, NA 0.8) on HeLa and RPE1 p53-/- cells, where 6 high-resolution images at 2720x2720 pixels were annotated with 582 objects.

    Imaging:

    Article Title: Listeria-infected macrophages promote biomechanical alterations in endothelial cell monolayers for transmigration.
    Article Snippet: Thirty minutes before the TFM recording, cell culture media was changed to L-15 CO 2 -independent medium supplemented with EGMTM-2 BulletKit Medium (MEDIUM CC-3156 and Kit CC-4176, Lonza). .. Samples in the 24-well plate were then transferred to the insert stage of an inverted Nikon Eclipse Ti2 epifluorescence microscope equipped with a Prime 95B camera for imaging. .. Note that for experiments involving addition of recombinant TNF-α (10 ng/mL, DL20083053, Invivogen) or anti-TNF-α antibody (1 μg/mL, MAB9677, Bio-techne) or isotype control (1 μg/mL, DNP-M2, ACROBiosytems), treatments were applied at the same time as MΦ.

    Plasmid Preparation:

    Article Title: Vector-free DNA transfection by nuclear envelope mechanoporation.
    Article Snippet: .. Plasmid transfection was also observed with an epifluorescence inverted microscope (Nikon Eclipse Ti2 Inverted microscope, Melville, NY), and images were captured using a digital camera (Hamamatsu C11450 ORCA Flash-4.0LT, Bridgewater, NJ). ..

    Transfection:

    Article Title: Vector-free DNA transfection by nuclear envelope mechanoporation.
    Article Snippet: .. Plasmid transfection was also observed with an epifluorescence inverted microscope (Nikon Eclipse Ti2 Inverted microscope, Melville, NY), and images were captured using a digital camera (Hamamatsu C11450 ORCA Flash-4.0LT, Bridgewater, NJ). ..

    Inverted Microscopy:

    Article Title: Vector-free DNA transfection by nuclear envelope mechanoporation.
    Article Snippet: .. Plasmid transfection was also observed with an epifluorescence inverted microscope (Nikon Eclipse Ti2 Inverted microscope, Melville, NY), and images were captured using a digital camera (Hamamatsu C11450 ORCA Flash-4.0LT, Bridgewater, NJ). ..

    Article Title: Nanodomain Formation and Temperature-Dependent Diffusion in Deep Eutectic Solvents Revealed by Single-Molecule Tracking.
    Article Snippet: Deep eutectic solvents (DESs) are typically regarded as homogeneous liquids; however, recent work shows that many exhibit nanoscale structural heterogeneity.. Most studies attribute these nanoscale features to short-range chemical interactions.. It is still unclear whether a long-range physical mechanism also plays a role.

    Article Title: Thioredoxin-mimetic peptide attenuates epilepsy progression and neurocognitive deficits
    Article Snippet: .. Live-cell imaging was conducted with a 20 × fluorite objective of an epifluorescence inverted microscope (Nikon ECLIPSE Ti2, Nikon Corporation, Japan) equipped with a xenon arc lamp (Cairn Research, Kent, UK) and a cooled CCD camera (Retiga; QImaging). ..

    Article Title: Gene transcription and chromatin packing domains form a self- organizing system
    Article Snippet: .. For widefield epifluorescence, all conditions were imaged using the Nikon Eclipse Ti2 inverted microscope equipped with a Prime 95B Scientific CMOS camera. ..

    Generated:

    Article Title: mnDINO: Accurate and robust segmentation of micronuclei with vision transformer networks
    Article Snippet: .. MNFinder images are taken from both Leica DMi8 laser scanning confocal microscope and Leica DMi8 with Adaptive Focus widefield microscope at 20x and 40x resolutions. mnDINO data subset 01 is generated from a Nikon Ti2 Eclipse inverted epifluorescence microscope, equipped with a IRIS9 camera, a 20X objective (Plan Apochromat, NA 0.8) and a 0.7X demagnifying C-Mount adaptor using HeLa cells, resulting in 18 high-resolution images of 2960x2960 pixels. .. 1,688 ground truth objects were manually annotated. mnDINO data subset 02 comes from a Nikon Ti2 Eclipse epifluorescence microscope, equipped with a Kinetix camera and a 20x objective (Plan Apochromat, NA 0.8) on HeLa and RPE1 p53-/- cells, where 6 high-resolution images at 2720x2720 pixels were annotated with 582 objects.

    Live Cell Imaging:

    Article Title: Thioredoxin-mimetic peptide attenuates epilepsy progression and neurocognitive deficits
    Article Snippet: .. Live-cell imaging was conducted with a 20 × fluorite objective of an epifluorescence inverted microscope (Nikon ECLIPSE Ti2, Nikon Corporation, Japan) equipped with a xenon arc lamp (Cairn Research, Kent, UK) and a cooled CCD camera (Retiga; QImaging). ..



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